Janet Barnette
Laboratory Technician
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Workphone: 801-581-5927
Office Address: ASB 520

Education
Bachelor of Science, University of Utah, 1998



Research interests
After graduating from the University of Utah in 1998, I joined Mark Keating's molecular biology lab at the University of Utah. I worked under the guidance of Alex Nechiporuk, a graduate student, and Kenneth Poss, a post-doc. I was hired to manage a budding zebrafish facility and serve as technician to Alex. My bench work focused on zebrafish fin regeneration. I became expert at cryo-sectioning zebrafish fins.

In 2000, Mark Keating moved his laboratory to Boston. Although I seriously considered moving across the country to try something different, the thought of finding an affordable Boston apartment that would take 3 cats was too daunting, so I stayed in Utah.

I moved on to a developmental biology lab at the Hunstman Cancer Institute. I was hired by Joseph Yost and worked for Kenneth Kramer, a post-doc, for three amazing years. For the most part, I worked with Xenopus embryos and studied left-right development. It was in this lab that learned to micro-inject frog embryos with precision and speed, earned authorship on a Cell paper, and found my first love, the Western blot.

A couple of years after joining the Yost Lab, I met and married my second love, Phil Barnette, a physician dabbling in science while completing his Pediatric Hematology/Oncology fellowship.

Although things were going well, I couldn't let go of the interests in Ecology, Evolution and Stable Isotopes that I had savored while in college, so I began to look for a job in the Biology department. After over a year of applying for jobs, I finally had the opportunity I'd been waiting for, and that was to come to work for Jim Ehleringer.

I joined Jim's lab in November 2003. I was hired to work on a 2-year forensic-related project. When that projected ended, I spend the following year working on a microbiology project, under the guidance and direction of Helen Kreuzer-Martin (currently at Pacific Northwest National Laboratory) looking at different strains of microbes grown on various media created with isotopically enriched waters. Currently, I'm back to forensics work, splitting my time between it and a water project looking at nitrates (concentration and 15N) in urban and non-urban ecosystems. I have keen interests in molecular biology, forensics, and biogeography.

I'm a 3/4-time working mother of three, and am grateful for the opportunity Jim has given me to have a family and a career.  I look forward to the next challenge!


Publications
Regulation of Casein Kinase I{epsilon} Activity by Wnt Signaling*
Wojciech Swiatek, I-Chun Tsai‡, Laura Klimowski, Andrea Pepler, Janet Barnette, H. Joseph Yost, and David M. Virshup
J. Biol. Chem., Vol. 279, Issue 13, 13011-13017, March 26, 2004

PKCg Regulates Syndecan-2 Inside-Out Signaling during Xenopus Left-Right Development
Kenneth L. Kramer, Janet E. Barnette, and H. Joseph Yost
Cell, Volume 111, Issue 7, 27 December 2002, Pages 981-990


Assessment of Polymorphism in Zebrafish Mapping Strains
Alex Nechiporuk, Janet E. Finney, Mark T. Keating, and Stephen L. Johnson.
Genome Res. 1999 December; 9(12): 1231–1238.